Grey, who currently plays herself on HBO’s Entourage, appears in a six-page nude pictorial along with a short interview.
Playboy’s October issue streets on Friday in both traditional and digital formats.
Grey’s previous appearance in Playboy came via a six-page spread in December 2009 in which she paid homage to the 1960s film Lolita.
Grey’s latest projects include producing Frankie Latina’s Modus Operandi, which opened in New York in early September, and finishing her first book, Neü Sex, later this fall.
The mainstream crossover film star’s role on Entourage where she plays the love interest of Vincent Chase (Adrien Grenier) was a dominant plotline this season. Her story arc continues into next season, the show’s final run.